Project Outcomes - Year 3
Year 3: Period 1
Projects completed in the first period of Year 3. Addressing requests recieved during the second half of Year 2. See: Proposals Received
Includes data releases: Y3: 1621
| SSG Rank | Task | Outcome |
|---|---|---|
| 2.25 | Axon extension of Martinotti cells | Added 56 cells |
| 2.25 | Axon backtrace in V1 column | Cleaned 448 axons, attached 163 spineheads |
| 3.25 | Axon extension of coregisted cells | Added 26 cells |
| 3.5 | Proofreading of microglia | Cleaned 60 cells |
| 5.5 | Axon extension of layer 1 neurogliaform cells | Added 10 cells |
| declined | Dendrite cleaning of L5-ET | Analysis support provided |
Axon extension of Martinotti cells
Proofreading Martinotti cells in all layers in the expanded column, a 50 um radius around the V1 column. Added 56 Martinotti cells across all layers. Available with data release 1621
Queried from CAVE table: proofreading_status_and_strategy:
# Standard query
client.materialize.query_table('proofreading_status_and_strategy')
# Content-aware query
client.materialize.tables.proofreading_status_and_strategy(status_axon='t').query()Axon backtrace in V1 column
Axon backtracing of inputs to cortical minicolumns produced two tables to densely proofread inputs to a set of apical axons: 1) a table annotating the backtracing effort, and 2) a table quantifying the number of disconnected synapses in a small area and the outcome of evaluating the synapse as a disconnected spine head.
Outcome: * annotated and proofread 448 axons passing through the V1 column, annotated in vortex_axon_backtrace_column * also attached 163 disconnected spineheads, annotated in vortex_synapse_reattachment
Axon extension of coregisted cells
Axon proofreading of high-responding functionally coregistered cells, cells identified by the requester. Added 26 fully extended coregistered cells with data release 1621
Queried from CAVE table: proofreading_status_and_strategy:
# Standard query
client.materialize.query_table('proofreading_status_and_strategy')
# Content-aware query
client.materialize.tables.proofreading_status_and_strategy(status_axon='t').query()Proofreading of microglia
Manual screening and proofreading of microglia within the V1 column. Added 60 cleaned and extended microglia within the V1 column.
Queried from CAVE table: vortex_microglia_proofreading_status:
# Standard query
client.materialize.query_table('vortex_microglia_proofreading_status')
# Content-aware query
client.materialize.tables.vortex_microglia_proofreading_status(status='clean').query()
Axon extension of layer 1 neurogliaform cells
Cleaning and extension of neurogliaform cells in and around the V1 column.
The 10 completed cells are included as axon partially extended. Although every effort was made to extend the cells to completion within the volume, highly interrupted segmentation in the superficial layer 1 made full extension intractable.
Queried from CAVE table: proofreading_status_and_strategy:
# Standard query
client.materialize.query_table('proofreading_status_and_strategy')
# Content-aware query
client.materialize.tables.proofreading_status_and_strategy(status_axon='t').query()